Analysis of predicted targets of miR-1195. (A) Venn diagram of the intersection of in silico miR-1195 predicted target genes in TargetScanMouse 6.2 and genes upregulated in Nkx2-1 knockdown cells identified by microarray analysis. (B) Expression level of Nkx2-1 and of the 4 predicted targets of miR-1195 in Nkx2-1shRNA treated MLE15cells vs. non-silencing control, determined by microarray analysis. FC, fold change; (1, 2, 3) biological replicates; red represents higher expression levels; green represents lower expression levels. (C) The 4 predicted targets of miR-1195 were validated by RT-qPCR in Nkx2-1 shRNA treated cells vs. non-silencing control MLE15 cells. (*) p < 0.05. (D) A miR-1195 inhibitor transduced in MLE15 cells reduces the expression of miR-1195 by 50% determined by RT-qPCR; expression of miR-1195 predicted target genes in the same cells inversely correlated to the levels of miR-1195 determined by RT-qPCR. (*) p < 0.05.