Analysis of predicted targets of miR-200c. (A) Venn diagram showing the intersection analysis of in silico miR-200c predicted target genes in TargetScanMouse 6.2 and genes downregulated in Nkx2-1 knockdown cells identified by microarray analysis. (B) Expression level of Nkx2-1 and of the 32 predicted targets of miR-200c identified in the intersection in A, determined by microarray analysis. FC, fold change; (1, 2, 3) biological replicates; red represents higher expression levels; green represents lower expression levels. Adjusted p value < 0.0005 (C) Selected transcription factors target of miR-200c were validated by RT-qPCR in Nkx2-1 shRNA treated cells vs. non-silencing control MLE15 cells. N = 3-6. (*) p < 0.01. (D) 3′UTR-Luciferase analysis of miR-200c predicted targets Myb, Nfib and Six1 compared to control-Luciferase in MLE15 transfected with pre-miR-200c or scrambled control. (*) p < 0.05.