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Figure 2 | Respiratory Research

Figure 2

From: Transcription factor and microRNA interactions in lung cells: an inhibitory link between NK2 homeobox 1, miR-200c and the developmental and oncogenic factors Nfib and Myb

Figure 2

Nkx2-1 controls the expression of specific miRNAs. A. Hierarchical two-dimensional clustering analysis of Nkx2-1-regulated miRNA expression in MLE15 cells. Blue, Non-silenced Nkx2-1 samples; yellow, Nkx2-1-shRNA knocked-down samples. Each row corresponds to a miRNA and each column corresponds to an independent sample. Green represents lower and red higher relative expression. B. RT-qPCR validation analysis of miRNAs down- regulated (upper panels) or up-regulated (lower panels) by Nkx2-1 knockdown in MLE15 cells. Data was normalized to rnuRNA-6B. (*) p < 0.05. C. RT-qPCR analysis of miRNA expression levels in Nkx2-1PM/PM relative to Nkx2-1+/+. Data was normalized to snoRNA-202 and represented in a log scale. (*) p < 0.05.

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Respiratory Research

ISSN: 1465-993X

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