Figure 4

Quantification of α-SMA positive cells by flow cytometry. Flow cytometry for α-SMA (% positive) in HLF Alone, HLF Co-Cultured with Heathy BECs, and HLF Co-Cultured with Heathy BECs following 96 hr exposure. Flow cytometry revealed 3.6% α-SMA positive cells in the healthy co-culture group compared to 8.1% α-SMA positive cells in the asthmatic co-culture group (p < 0.03) and 11.6% α-SMA positive cells in the HLF alone (p < 0.03).