Figure 3

Flow cytometric analysis of apoptosis induction and cell cycle distribution in A549 and 16HBE cells. A) A549 cells were treated with denatonium (1 mM or 2 mM) for 72 h, stained with FITC-annexin V/PI and then analyzed by flow cytometry. The right panel shows the apoptosis rates of the cells of the various groups. Flow cytometry was also used to analyze DNA at the G1, S, and G2 phases of the cell cycle. B) 16HBE cells were treated with denatonium (0.5 mM, 1 mM or 2 mM) for 24 h. Then the number of apoptotic cells and the number of cells in different stages of the cell cycle were detected by flow cytometry. Data are representative of three similar experiments. **indicates significant difference at p < 0.01 versus control and ***indicates significant difference at p < 0.001 versus control.