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Figure 2 | Respiratory Research

Figure 2

From: Expression of surfactant protein D in airways of asthmatics and interleukin-13 modulation of surfactant protein D in human models of airway epithelium

Figure 2

Surfactant protein D expression ALI cultures. SP-D expression was examined in ALI sections grown from non-asthmatic and asthmatic donors via immunohistochemistry, where pink was indicative of positivity [A]. Fraction of total epithelial area positive was quantified using colour segmentation in ImagePro Plus. Expression of SP-D in the ALIs of asthmatic AEC was significantly higher compared to that of non-asthmatic AEC (n = 5, t-test p = 0.04) [B]. SP-D protein expression in total cell lysates of ALI cultures was analyzed by Western blot. Expression of SP-D was quantified by densitometry using ImageJ and normalized to β-actin. ALIs of asthmatic AEC demonstrated significantly higher expression of SP-D compared to that of non-asthmatic AEC (n = 3-7, t-test p = 0.005) [C].

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