Figure 3

Dietary intervention with 1% GOS reduced pulmonary eosinophilic inflammation in the lungs of HDM allergic mice. Infiltration of inflammatory cells in the BALF of house dust mite allergic mice. HDM-PBS: HDM- sensitized and PBS-challenged mice (white bars), HDM-HDM: HDM-sensitized and -challenged mice (grey bar). Contr: control diet, GOS: 1% GOS diet, Bud: budesonide treatment. Total BAL cells (A), absolute number of eosinophils (B), lymphocytes (C), macrophages (D) and neutrophils (E). Results are shown as mean ± SEM. Statistical significance of differences was tested using post hoc Bonferroni’s multiple comparisons test after One-Way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, n = 7–9 mice/group.