Figure 1

Time schedule of the house dust mite allergic asthma model. Male BALB/c mice were sensitized intranasally (i.n.) with house dust mite (HDM) on day 0 and were challenged on days 7 to 11 i.n. with HDM or PBS. Mice were fed control diet (AIN93G, contr) or 1% v/w GOS from day −14 to 14. In a separate set of animals, control mice and HDM-allergic mice were oropharyngeally instilled with budesonide on days 7, 9, 11 and 13. All mice were sacrificed on day 14.