Figure 4

IL-27 signaling affects IL-10 production during influenza, S. aureus co-infection. C57BL/6, WT and IL-27Rα−/− mice received 100 PFU of influenza A PR/8/34 or vehicle (PBS) for 6 days followed by 1x10⁸ of S. aureus (ATTC 49775) or vehicle (PBS) for 24 hours. Type 17-associated inflammatory cytokine mRNA gene expression was measured in lung homogenate by RT-PCR, n = 7 (A) and cytokine/chemokine levels in lung homogenate were measured by Lincoplex, n = 7 (B-G). IL-10 mRNA expression in lung homogenate as measured by RT-PCR (H). Data are the combined results of two replicate experiments. *p < 0.05 IL-27Rα−/− mice infected with S. aureus versus influenza, S. aureus. **p < 0.05 C57BL/6 mice infected with S. aureus versus influenza, S. aureus. ∞p < 0.05 IL-27Rα−/− mice infected with S. aureus versus C57BL/6 mice infected with S. aureus. ♦p < 0.05 IL-27Rα−/− mice co-infected with influenza, S. aureus versus C57BL/6 mice co-infected with influenza, S. aureus. All data are reported as means ± SEM for each group by unpaired t test.