Figure 5
From: Regulation of IL-17A responses in human airway smooth muscle cells by Oncostatin M
Receptor chain mRNA levels in stimulated HASMC. HASMC were plated and prepared for RNA analysis as described in methods after 6 hours of stimulation with the indicated cytokines at 5 ng/ml or 2 ng/ml OSM (n = 2). RNA was prepared and analyzed by qRT-PCR using probes for the receptor subunits IL-17RA, IL-17RC, OSMRβ, gp130/IL-6ST and IL-4Rα, as indicated. Levels are expressed as fold change relative to unstimulated (control) and corrected to β-Actin as an endogenous reference control.