Figure 4

Effect of TGFβ1 on lung morphometry. (A) Representative images of lung histology (H&E stain) of NB TGFβ1TG mice and TGFβ1TG X TGFβR2KO mice. Scale bar: 100 μm (B) Bar graph showing the mean chord length in TGFβ1TG mice and TGFβ1TG X TGFβR2KO mice. (C) Bar graph showing the septal thickness in TGFβ1TG mice and TGFβ1TG X TGFβR2KO mice. (D) Bar graph showing the radial alveolar count in TGFβ1TG mice and TGFβ1TG X TGFβR2KO mice. (E) Bar graph showing the secondary septal crest numbers in TGFβ1TG mice and TGFβ1TG X TGFβR2KO mice. (F) Representative images of lung immunohistochemistry of vessel density (vWF staining) of NB TGFβ1TG mice and TGFβ1TG X TGFβR2KO mice. Scale bar: 100 μm (G) Bar graph showing quantification of vessel density in TGFβ1TG mice and TGFβ1TG X TGFβR2KO mice. N = 4, in each group; *p < 0.05, **p < 0.01.