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Figure 4 | Respiratory Research

Figure 4

From: Increased constitutive αSMA and Smad2/3 expression in idiopathic pulmonary fibrosis myofibroblasts is KCa3.1-dependent

Figure 4

Basal nuclear total Smad2/3 expression is significantly attenuated by K Ca 3.1 blockers. (A) Constitutive nuclear Smad2/3 expression was significantly attenuated by the KCa3.1 blocker TRAM-34 (200 nM) (NFC n = 5 [triangles] and IPF n = 6 [circles] (data pooled for statistical analysis, at least 10 cells were measured per donor). (B) Immunofluorescent images depict the reduced nuclear staining following 1 hour of incubation with TRAM-34. (C and D) Similarly, ICA-17043 (100 nM) significantly reduced the proportion of total Smad2/3 staining within the nuclei of HLMFs after a 1 hour incubation. Results are represented as mean ± SEM, *P < 0.05 (Paired t-test).

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