Figure 4

The involvement of HO-1 in the suppressive effects of quercetin on LPS-induced pro-inflammatory cytokine production. AMJ2-C11 cells were cultured with quercetin (20 μM) for 1 hour after treatment of SnPP (20 μM) for 30 minutes, and then stimulated with LPS (5 μg/ml) for 2 hours to detect the mRNA expression of TNF-α, IL-1β, and IL-6 determined by quantitative real-time PCR (A), and for 18 hours to detect the production of TNF-α, IL-1β, and IL-6 determined by ELISA (B), respectively. Results are shown as the mean ± SD (*p < 0.05) of three independent experiments.