Figure 8
From: Vitamin D regulating TGF-β induced epithelial-mesenchymal transition
Calcitriol inhibits the migration of TGF-β stimulated BEAS-2B cells. BEAS-2B cells were stimulated with 100 nM calcitriol for 24 hours followed by 48 hours of stimulation by 10 ng/ml of TGF-β1 or TGF-β2. A: Representative images of a wound healing scratch assay. Pictures of the same area were taken at 0 and 48 hours at 20x magnification. The area of the wound was measured using the NIH ImageJ program. B: The % wound closure was calculated and means of groups were compared by one-way ANOVA. Data is presented as mean × SEM (n =4), *p <0.05 and **p <0.01 compared to control, ##p <0.01 compared to calcitriol treated cells, §p <0.05, §§p <0.01.