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Figure 6 | Respiratory Research

Figure 6

From: Vitamin D regulating TGF-β induced epithelial-mesenchymal transition

Figure 6

Calcitriol prevents TGF-β-mediated EMT: MMP2 and MMP9 mRNA and protein expression changes in BEAS-2B cells. BEAS-2B cells were stimulated with the active form of Vitamin D, calcitriol (100 nM). After 24 hours, TGF-β1 (10 ng/ml) or TGF-β2 (10 ng/ml) was added to the cells for an additional 48 hours. Total RNA was isolated and assessed for the expression of A: MMP2 (n =4) and B: MMP9 (n =4) by means of quantitative real-time PCR. Expression levels were normalized to the housekeeping 18 s and calculated as mean level of induction in comparison to 0.1% vehicle control cells. Data is presented as mean × SEM *p <0.05, **p <0.01, and ***p <0.001 compared to control, #p <0.05, ##p <0.01, and ###p <0.001 compared to calcitriol, §p <0.05, §§p <0.01, and §§§p <0.001 by one-way ANOVA. C: Conditioned supernatant of BEAS-2B cells stimulated with TGF-β1, TGF-β2 and/or 100 nM calcitriol were subject to gelatin zymography. Results are representative of 3 separate experiments.

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