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Figure 3 | Respiratory Research

Figure 3

From: Vitamin D regulating TGF-β induced epithelial-mesenchymal transition

Figure 3

mRNA expression changes in BEAS-2B cells upon calcitriol time course and dose response. Calcitriol (100 nM) was added to BEAS-2B cells for 2, 6, 12, 24 and 48 hours. Total RNA was isolated and analyzed for the expression of E-cadherin by qPCR. Expression levels were normalized to the housekeeping gene 18 s and calculated as mean level of induction in comparison to control untreated cells (A). Data is presented as mean × SEM n =3, *p <0.05, by one-way ANOVA. For calcitriol dose response, response treatment BEAS-2B cells were stimulated with increasing doses of calcitriol starting from 0.1 nM and up to 100 nM for 24 hours. Total RNA and protein were isolated and assessed for the expression of E-cadherin (B). Data is presented as × SEM n =3, *p <0.05, **p <0.01 one-way ANOVA.

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