Figure 1

mRNA and protein expression changes in BEAS-2B cells upon TGF-β1 dose response treatment. BEAS-2B cells were stimulated with increasing doses of TGF-β1 starting from 0.1 ng/ml and up to 50 ng/ml for 72 hours. Total RNA and protein were isolated and assessed for the expression of A: E-cadherin, B: Snail, C: Vimentin, D: N-cadherin, and by means of quantitative real-time PCR and western blot respectively. Protein and mRNA expression levels were normalized to the housekeeping GAPDH and calculated as mean level of induction in comparison to control untreated cells. Data is presented as mean × SEM n =4, *p <0.05, **p <0.01, and ***p <0.001 by one-way ANOVA.