Figure 8
From: Surfactant protein D attenuates sub-epithelial fibrosis in allergic airways disease through TGF-β
SP-D regulates eosinophil-derived TGF-β1 and IL-13. Effect of SP-D on eosinophils-derived TGF-β1 and IL-13 production was examined in vitro experiment. (A): Level of total TGF-β1 in eosinophil culture supernatant. (B): Level of total TGF-β1 in supernatant of eosinophil cultures in the presence of normal and heat inactivated SP-D. (C): Level of IL-13 in eosinophil culture supernatant. Eosinophils from IL-5 transgenic mice were incubated in vitro with Dp for 24 hr in the presence or absence of SP-D pre-incubation as described Material and Methods. TGF-β1 and IL-13 in culture supernatants was determined by ELISA. Data are presented as means ± SEM obtained from 2 different experiments (N = 6). White bars: control. Black bars: Dp stimulation at different concentrations. Shaded bars: Dp stimulation with SP-D pre-incubation at different concentrations. *p < 0.05.