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Figure 7 | Respiratory Research

Figure 7

From: Surfactant protein D attenuates sub-epithelial fibrosis in allergic airways disease through TGF-β

Figure 7

Eosinophil infiltration and TGF-β1 and IL-13 production in lungs of Dp-challenged mice. (A) Photomicrographs of Luna staining (eosinophils) and immunohistochemistry for TGF-β1 and IL-13. Photographs were representative in 3 different experiments. Magnification: 400x. Scale bar =50 μm. Sequential staining were performed between Luna staining and immunohistochemistry for TGF-β1and IL-13. Blue arrows: TGF-β1 positive eosinophils; Red arrows: IL-13 positive eosinophils. Dp/PBS: Dp-sensitized and PBS-challenged mice. Dp/Dp: Dp-sensitized and Dp-challenged mice. (B) Morphological analysis of eosinophil infiltration in lungs Eosinophils under the sub-epithelial region were counted as described in Material and Methods. Data are presented as mean ± SEM obtained from 3 different experiments. 3–5 mice per group were used in each experiment (N = 7-11). White bars: PBS challenged mice in each groups. Black bar: Dp challenged WT mice. Shaded bar: Dp challenged SP-D−/− mice. Dotted bars: Dp challenged SP-D−/− mice with SP-D rescue. *p < 0.05.

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