Figure 6

BAL fluid cell analysis and cytokine concentration in Dp-challenged mice with treatment of SP-D. (A) BAL was performed for total cell count, macrophages and eosinophils. (B); Level of total TGF-β1 in BAL fluid. (C); level of active TGF-β1 in lung homogenate. (D); level of IL-13 in lung homogenate. Cytokines in BAL and lung homogenates was measured by ELISA. Data are presented as means ± SEM obtained from 3 different experiments. 3–5 mice per group were used in each experiment (N = 7-13). White bars: PBS challenged mice in each groups. Black bar: Dp challenged WT mice. Shaded bar: Dp challenged SP-D−/− mice. *p < 0.05.