Figure 4

Th2/Th1 cell population in Dp challenged mice. Th2/Th1cell population were examined by flow cytometry. (A): Gating information and contour plots of CD3+ CD4+ lymphocytes from lung digests, among the 4 mice groups. Lymphocytes were initially examined for dual expression of CD3 and CD4. Data was representative in 3 different experiments, with n = 3 to 5 mice per condition. (B): IL-4 positive Th cells population. (C): IFN-γ positive Th cells population. IL-4/IFN-γ positive cells were analyzed in CD3 + CD4 T cells. Percent of intracellular cytokine positive cells relative to CD3 + CD4+ T cells of lung digests were analyzed by flow cytometry. Data were representative across 3 independent experiments and were presented as means ± SEM of 3–5 mice per group. White bars: PBS challenged mice in each groups. Black bar: Dp challenged WT mice. Shaded bar: Dp challenged SP-D−/− mice. (D): mean fluorescence intensity (MFI) of IL-4 per T cell basis. (E): MFI of IFN-γ per cell basis. per cell basis. Data was representative in 3 different experiments, with n = 3 to 5 mice per condition. Stippled line: negative control IgG. Blue solid line: PBS challenged mice. Red bold line: Dp challenged line. MFI: mean fluorescence intensity.