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Figure 6 | Respiratory Research

Figure 6

From: Small interfering RNA against CD86 during allergen challenge blocks experimental allergic asthma

Figure 6

Lack of effect of treatment with CD86 siRNA on mucus production in OVA-challenged mice. (A) Lungs were obtained 36 hours after the last OVA challenge and inflated in formalin. Sections were stained with AB/PAS to identify mucus-containing cells. Representative sections from naïve, control siRNA, and CD86 siRNA-treated mice are shown. (B) Semi-quantitative analysis of the abundance of PAS-positive cells. The numeric scores for the abundance of PAS-positive, mucus-containing cells in each airway were determined to be as follows: 0, <5% PAS-positive cells; 1, 5–25%; 2, 25–50%; 3, 50–75%; 4, >75%. (C) MUC5AC expression in mouse whole lung was analyzed by real-time PCR. The relative levels of the MUC5AC transcripts were presented as fold increase over baseline values. β-actin was taken as a house-keeping gene. All data mean ± SEM of 6–10 mice per group. n.s. means statistically not significant.

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