Figure 4
From: Small interfering RNA against CD86 during allergen challenge blocks experimental allergic asthma
Effect of siRNA treatment on CD86 positive cells around airway. Sensitized mice were intratracheally administered with CD86 siRNA or control siRNA and given a single challenge with OVA. Twelve hours after the challenge, the tracheas and lungs were removed and processed for immune-fluorescence study. Naïve mice served as negative control. (A) Lung sections from naïve, CD86 siRNA-treated or control siRNA-treated mice stained with FITC-conjugated anti-CD86 mAb. Bar, 200 μm for upper panel; 100 μm for lower panel. The upper panel and lower panel were obtained from different mice (B) Micrographs were quantitatively analyzed for the presence of FITC-anti-CD86 signals per unit area with image analysis software. All data mean ± SEM of 3-4 mice per group. * indicates statistically significant (P <0.05) difference between the indicated two groups.