Figure 2
From: Small interfering RNA against CD86 during allergen challenge blocks experimental allergic asthma
Th2 cytokine levels in culture supernatant were analyzed by ELISA. (A) DO11.10 spleen cells were primed with pOVA and APCs in the presence of rIL-4, anti-IL-12 antibody, CD28, and IL-2. Seven days later, CD4-positive cells were purified using MACS and used as effector T cells. CD86 siRNA-transfected BMDCs were pulsed with pOVA and stimulated with LPS and then cultured with effector T cells. (B) Coculture supernatants were collected 48 hours later, and cytokine levels were measured by ELISA (n = 4; mean ± SEM). * indicates statistically significant (P <0.05) difference between the indicated two groups.