Figure 7

Pro-inflammatory cytokine expression was assessed per 15 μg total lung protein obtained from E18.5 pups by ELISA. TNF-α (A) and IL-1β (B) levels were both significantly increased in SHS-exposed wild type pup lungs compared to room air exposed controls. Cytokine levels were not different in lungs from wild type and RAGE KO mice exposed to room air. Lung samples from SHS-exposed RAGE KO pups revealed significant decreases in SHS-induced cytokine expression when compared to SHS-exposed controls. *Statistical differences (P ≤0.05) with at least three replicates per group.