Figure 2

Significant up-regulation of RAGE was observed by immunofluorescence using sections of E18.5 mouse lungs in pups from time mated pregnant wild type dams (A and B) and age-matched RAGE KO animals (C and D). Specifically, red RAGE immunofluorescence was increased in wild type lungs exposed to SHS (B) compared to room air controls (A) and no expression was detected in RAGE KO animals exposed to room air (C) or SHS (D). Images were at 100X original magnification and scale bars equal 100 nm. Immunoblotting using 10 μg of total E18.5 lung protein revealed marked up-regulation of RAGE in lungs exposed to SHS compared to room air controls (E). Densitometry of RAGE in the blots revealed an approximate 100% increase in protein expression (F). Immunoblotting data are representative of experiments performed in triplicate.