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Figure 7 | Respiratory Research

Figure 7

From: MicroRNA-708 regulates CD38 expression through signaling pathways JNK MAP kinase and PTEN/AKT in human airway smooth muscle cells

Figure 7

Effect of miR-708 transfection on PTEN/AKT signaling. A, B and C: NA-HASM cells were transfected with 50 nM miR-708 mimic (mimic) or scrambled sequence mimic (scr) and treated with rh-TNF-α for 2 h to measure total and phosphorylated AKT (n = 3 donors) or 24 h to measure AKT2 (n = 3 donors) and PTEN expression (n = 8 donors) by Western blot analysis of cell lysates. Note significant down-regulation of AKT phosphorylation and AKT2 expression and up-regulation of PTEN expression. D: PTEN expression in AS-HASM cells was higher (not significant) in samples from 3 of 4 donors. Each Western blot is a representative blot from a single donor. Values are means ± SEM for results shown. C-vehicle-treated cells (0.1% BSA); T-TNF-α (10 ng/ml) treated cells.

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