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Figure 6 | Respiratory Research

Figure 6

From: MicroRNA-708 regulates CD38 expression through signaling pathways JNK MAP kinase and PTEN/AKT in human airway smooth muscle cells

Figure 6

Effect of miR-708 transfection on JNK MAP kinase signaling in NA-HASM cells. A and B: Western blot analysis of cell lysates following transfection with 50 nM miR-708 mimic (mimic) or scrambled sequence mimic (scr) was performed as described in Figure 5 to detect JNK MAP kinase after rh-TNF-α treatment for 20 min (total and phosphorylated levels) (n = 4 donors) as well as total JNK expression after rh-TNF-α treatment for 24 h (n = 3 donors). Note significant down-regulation of JNK MAP kinase phosphorylation but no change in total JNK protein expression in mimic-transfected cells compared to cells transfected with scr. C: Total RNA extracted from cells transfected with mimic or scr and treated with rh-TNF-α (24 h) was analyzed for JNK mRNA expression by q-PCR (n = 3 donors). Note significant down-regulation of JNK expression by mimic. D: MKP-1 expression in cells transfected with mimic or scr and treated with rh-TNF-α for 24 h was examined by Western blot analysis (n = 5 donors). Note significant up-regulation of MKP-1 expression in mimic-transfected cells compared to scr. Each Western blot is a representative blot from a single donor. Values are means + SEM for results shown. C-vehicle-treated cells (0.1% BSA); T-TNF-α (10 ng/ml) treated cells.

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