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Figure 5 | Respiratory Research

Figure 5

From: MicroRNA-708 regulates CD38 expression through signaling pathways JNK MAP kinase and PTEN/AKT in human airway smooth muscle cells

Figure 5

Effect of miR-708 transfection on ERK and p38 MAP kinase activation and expression in NA-HASM cells. Western blot analysis of cell lysates following transfection with 50 nM miR-708 mimic (mimic) or scrambled mimic (scr) and treatment with rh-TNF-α for 20 min (for total and phosphorylated levels) or 24 h (for total expression levels) were performed with antibodies against phosphorylated and total ERK (A and B) as well as p38 (C and D), respectively. Note no significant change in expression of total or phosphorylated ERK (n = 5–6 donors) and p38 (n = 3–5 donors) following miR-708 mimic transfection. Each Western blot is a representative blot from a single donor. Values are means ± SEM for results shown. C-vehicle-treated cells (0.1%BSA); T- rh-TNF-α (10 ng/ml) treated cells.

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