Figure 2

MiR-708 inhibits CD38 expression in HASM cells. A: NA-HASM cells were transfected with different concentrations of miR-708 mimic (mimic) or scrambled sequence mimic (scr), followed by exposure to 10 ng/ml rh-TNF-α. CD38 transcript levels were measured by q-PCR. Results are shown as % CD38 mRNA relative to scrambled sequence mimic (scr) transfection. Significant inhibition of CD38 expression was observed following transfection of miR-708 mimic at concentrations ≥ 50 nM (n = 3–5 donors). B: CD38 transcript levels following transfection with miR-708 mimic (50 nM) or scrambled sequence mimic in AS-HASM cells. Note significant attenuation of CD38 expression by miR-708 mimic transfection (n = 3 donors); C: ADP-ribosyl cyclase activity in AS-HASM (AS) and NA-HASM (NA) cells following transfection with miR-708 mimic or scrambled sequence mimic and exposure to rh-TNF-α (MT and ST, respectively, (n = 3 donors). Note significant attenuation of enzyme activity in cells from AS and NA after transfection with miR-708 mimic. D: CD38 transcript levels in NA-HASM cells following transfection with miR-708 mimic (50 nM), scrambled sequence mimic or antagomir for the miRNA (ant-mir) (n = 3 donors). Note the lack of inhibition of CD38 expression in cells transfected with ant-mir. Data represents mean ± SEM.