NE-induced PlGF expression and secretion are mediated by Egr-1. (A-C) The mRNA level of PlGF was determined by reverse-transcriptional polymerase chain reaction (RT-PCR) with primer sets for PlGF and GAPDH cDNA. (D-F) Cellular lysates were also subjected to Western blot analysis with antibodies for PlGF and β-actin. (G-I) The PlGF in the culture medium was detected by enzyme-linked immuno-sorbent assay (ELISA). Data are presented as mean ± SEM. *p < 0.05 vs. vehicle-treated group.