NE-induced PlGF promoter activity is mediated by Egr-1. (A, E, and F) The placenta growth factor (PlGF) promoter activity was evaluated by luciferase activity. (B) Hypoxia inducible factor (HIF)-1alpha and metal-regulatory transcription factor (MTF)-1, (C) Egr-1, and β-actin were analyzed by Western blot analysis. (D) The association of Egr-1 and PlGF promoter fragment was evaluated by chromatin immuno-precipitation assay. Data are presented as mean ± SEM. *p < 0.05 vs. vehicle-treated group.