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Figure 3 | Respiratory Research

Figure 3

From: Roxithromycin treatment inhibits TGF-β1-induced activation of ERK and AKT and down-regulation of Caveolin-1 in rat airway smooth muscle cells

Figure 3

The time course of ERK and AKT activation in ASMCs stimulated with TGF-β1. (A, C) Asthmatic ASMCs were incubated with TGF-β1 (10 ng/ml) for 5, 10, 15, 20 and 60 min. The expression of p-ERK1/2 protein was assessed by western blot and analyzed by densitometry compared to ERK1/2 expression. The expression of p-ERK1/2 protein in ASMCs was increased and peaked at 20 min after TGF-β1 stimulation (*P < 0.05 versus control, 5, 10, 15, 60 min groups). (B, D) Asthmatic ASMCs were incubated with TGF-β1 (10 ng/ml) for 5, 10, 15, 20, and 60 min. The expression of p-AKT protein was assessed by western blot and analyzed by densitometry compared to AKT expression. The expression of p-AKT protein in ASMCs was increased and peaked at 20 min after TGF-β1 stimulation (*P < 0.05 versus control, 5, 10, 15, 60 min groups).

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