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Figure 2 | Respiratory Research

Figure 2

From: Smoking accelerates aging of the small airway epithelium

Figure 2

Expression of an aging gene signature in small airway epithelium of age matched healthy smokers vs healthy nonsmokers. A. Principal component analysis of gene expression of small airway epithelium of smokers (n = 29, orange circles) and nonsmokers (n = 29, green circles) using the de Magalhaes et al. [26] 67 gene aging signature as an input dataset. Data is corrected for all covariates except smoking status. B. Volcano plot, smoker vs nonsmoker small airway epithelium of the 67 aging gene signature. Ordinate - p value (log10); abscissa - fold-change (log2). Red circles represent genes significantly differentially expressed in smoker vs nonsmoker small airway epithelium (≥1.2 fold-change up- or down-regulated; p < 0.05 using a Benjamini-Hochberg correction of false discovery rate). C. Correlation analysis of the 18 aging genes differentially expressed in the small airway epithelium of healthy smokers vs healthy nonsmokers, comparing smoker vs nonsmoker fold-changes in U133 gene expression with U133 gene expression from an independent dataset (n = 12 nonsmokers and n = 10 smokers, GSE4498) [25]. D. Correlation analysis of the 18 aging genes differentially expressed in the small airway epithelium of healthy smokers vs healthy nonsmokers, comparing smoker vs nonsmoker fold-changes in U133 gene expression with RNA Seq gene expression from an independent dataset (n = 5 nonsmokers and n = 6 smokers) [27].

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