Cell migration and protein extravasation in BALF of SW mice 24 h after intra-tracheal challenge with ouabain (0.075 μmol/animal) or GLP (12 μg of GLP protein/animal). (A) Total cells, (B) neutrophils, and (C) total protein in BALF supernatant. Controls received 50 μL of sterile saline. Cell migration and edema formation in lungs of C57BL/10 and C57BL/10ScCr (TLR4-deficient) mice 24 h after intra-tracheal challenge with LPS (500 ng/animal) or GLP (12 μg of GLP protein/animal). Controls received 50 μL of sterile saline. Controls (white bars), LPS-treated (gray bars), and GLP-treated (black bars). (D) Total cells, (E) neutrophils and (F) total protein in BALF supernatants. The results are the means ± SEM of at least 8 animals. *P < 0.05 compared to the control group; #P < 0.05 when both strains were compared.