Figure 4

OVA/CFA sensitized mice have more γδ⁺IL-17⁺T cells in the BAL fluid. BAL fluid cells were stimulated with PMA/ionomycin and triple stained with α-CD4, α-γδ TCR and α-IL-17 antibodies. (A) Representative flow cytometry plots of cells in BAL fluid (top panel) and lung (bottom panel) gated first to identify IL-17⁺ cells and subsequently to identify frequencies of CD4 and γδ T cells. (B) The frequency distribution of IL-17⁺ cells within the CD4 and γδ T cell populations is presented as the ratio (γδ/CD4) of these cells. One-way ANOVA, Holm-Sidak. (C) Total numbers of CD4⁺IL-17⁺ and γδ⁺IL-17⁺ populations calculated from the frequency of these cells and the total cell counts are shown. Two-way ANOVA, Holm-Sidak. (D) The distribution of total γδ⁺IL-17⁺ and CD4⁺IL-17⁺ T cells is presented as the ratio (γδ/CD4) of these cells. One-way ANOVA, Holm Sidak. (B-D) Data are from 7–11 total mice per group from at least 2 independent experiments. *p < 0.05 comparisons between OVA groups (adjuvant-dependent effects), ^§p < 0.05 OVA sensitized groups versus respective controls (antigen-dependent effects).