Figure 1

Serum OVA-specific IgE and airway inflammatory responses are enhanced in OVA/CFA sensitized mice. BALB/c mice were IP OVA sensitized without adjuvant (OVA/sal), or in the presence of alum (OVA/alum) or CFA (OVA/CFA). Corresponding control groups were injected with saline, alum or CFA. (A) Serum OVA-IgE levels. OVA-IgE was undetectable in control mice. Mean (+SEM) from 8–12 mice per group from a minimum of 3 independent experiments. One-way ANOVA, Holm-Sidak. (B) BAL fluid differential cell counts from OVA sensitized groups (solid bars) and their respective adjuvant controls (striped bars). Mean total cells, macrophages, eosinophils, neutrophils and lymphocytes (+SEM) are shown for 11–16 total mice from at least 3 independent experiments. Two-way ANOVA, Holm-Sidak. (C) Relative expression of CCL2, CCL11, CCL24, CXCL1 and CXCL5 in the lung following OVA challenge assessed by real-time PCR. Expression is presented as fold increase relative to the saline control. Results represent the means (+SEM) from 5–6 total mice per group from a minimum of 2 independent experiments. Two-way ANOVA, Holm-Sidak. (A-C)^§p < 0.05 OVA sensitized groups vs. respective controls (antigen-dependent effects), *p < 0.05 comparisons between OVA groups (adjuvant-dependent effects).