Role of caspase 1 and 11 the expression of IL-1 family. Wild type, age matched control mice and GM mice (mice missing functional caspase 1/11 and caspase 11) were exposed to vehicle (aerosolised saline) or LPS (1 mg/ml) for 30 minutes. BALF was collected 6 hours after the exposure and IL-1α (A or D), IL-1β (B or E) and IL-18 (C or F) measured by ELISA. Data are represented as mean ± S.E.M. for n = 8 animals in each group. Statistical significance was determined using Mann–Whitney U test. # = P < 0.05, denoting a significant difference between the LPS exposed and vehicle exposed wild-type groups.