Role of TLR4 and Myd88 in the expression of IL-1 family and associated markers. Wild type, age matched control mice and GM mice (mice missing functional TLR4 or Myd88) were exposed to vehicle (aerosolised saline) or LPS (1 mg/ml) for 30 minutes. Lung tissue was collected 6 hours after the exposure. Lung tissue levels of mRNA expression were detected by RT-PCR NALP3 (A), Caspase 11 (B), IL-1α (C) and IL-1β (D). Data are represented as mean ± S.E.M. for n = 4 animals in each group. Statistical significance was determined using Mann–Whitney U test. # = P < 0.05, denoting a significant difference between the LPS exposed and vehicle exposed wild-type groups; * = P < 0.05, denoting a significant difference between the LPS exposed GM and wild-type mice (one-way ANOVA).