Expression of IL-1 family (protein levels). Male C57bl/6 mice were exposed to vehicle (aerosolised saline) or LPS (1 mg/ml) for 30 minutes. BALF was collected at different time points after the exposure and IL-1α (A), IL-1β (B) and IL-18 (C) measured by ELISA. Data are represented as mean ± S.E.M. for n = 6 animals in each group. Statistical significance was determined using Mann–Whitney U test. # = P < 0.05, denoting a significant difference between the LPS exposed and vehicle exposed wild-type groups.