Surfactant alteration and replacement in acute respiratory distress syndrome

Respiratory Research

Table 1 Phospholipid (PL) content and profile in patients with acute respiratory distress syndrome (ARDS) and in other subjects

	Hallman et al. [23]^a		Pison et al. [24]^b			Gregory et al. [25]^c			Günther et al. [26]^b
				Low-	High-						ARDS
				score	score						+
PL	Normal	ARDS	Normal	ARDS	ARDS	Normal	At risk	ARDS	Normal	ARDS	PNEU	PNEU
Total	0.6	0.6	84.1	65.7	65.1	7.99	3.48	2.47	29.38	28.11	22.57	21.82
	μM	μM	μg/ml	μg/ml	μg/ml	μmol/ml	μmol/ml	μmol/ml	μg/ml	μg/ml	μg/ml	μg/ml
PC	73	59.5	62.8	56.3	48.1	76.27	73.32	62.64^***	83.1	81.9	76.8	79.2
PG	12.4	0.3^***	10.02	1.6	1.88	11.58	7.26	6.48^***	8.6	3.5^***	2.4^***	5.2^**
PI	2.7	3.1	8.32	13.54	13.95	3.88	4.71	6.94^**	3.2	6.5^*	8.0^***	5.5^*
PE	2.6	4.3	4.82	13.65	18.68	3.32	4.9	5.86^*	1.7	1.9	2.6	2.4
PS	3.3	13.0^***	4.5	n.d.	n.d.	1.8	3.75	2.84	1.2	1.8	1.7	2.4
Sph	3.7	17.5^***	7.37	12.28	14.21	1.45	1.56	5.45^***	0.8	3.5^**	5.2^***	4.3^***
LPC	0.4	1.5	1.3	1.7	1.0	0.16	1.34	2.32^*	0.1	0.3	0.2	0.2

^aBALF post sucrose density gradient centrifugation at 100,000 × g (lipid-protein complex) was used. ^bOriginal BALF after cell separation (300 × g) was used. ^cCell-depleted BALF post centrifugation at 48,000 × g ('crude surfactant pellet') was used. The relative amount (mean values) of each PL is given as percentage of total PL. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. BLAF = bronchoalveolar lavage fluid; LPC = lysophosphatidylcholine; n.d. = not determined; PC = phosphatidylcholine; PE = phosphatidylethanolamine; PG = phosphatidylglycerol; PI = phosphatidylinositol; PNEU = pneumonia; PS = phosphatidylserine; Sph = sphingomyelin.

ISSN: 1465-993X