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Figure 4 | Respiratory Research

Figure 4

From: Collaborative interactions between neutrophil elastase and metalloproteinases in extracellular matrix degradation in three-dimensional collagen gels

Figure 4

Effect of prostaglandin E2 (PGE2) on matrix metalloprotease (MMP)-1. After four days, media were harvested for immunoblotting. (a) Human fetal lung (HFL) fibroblasts alone. Lane 1: control; lane 2: neutrophil elastase (NE); lane 3: indomethacin; Lane 4: PGE2. (b) Blood monocytes (BM) co-cultured with HFL fibroblasts. MMP-1 appears in its latent 52 kDa form. NE presence resulted in conversion of latent MMP-1 to the active 20 kDa form. Lane 1: control; lane 2: NE; lane 3: indomethacin; lane 4: NE + indomethacin; lane 5: PGE2; lane 6: NE + PGE2; lane 7: NE + indomethacin + PGE2. (c) BM alone. Lane 1: control; lane 2: NE; lane 3: indomethacin; lane 4: PGE2.

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