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Figure 3 | Respiratory Research

Figure 3

From: Collaborative interactions between neutrophil elastase and metalloproteinases in extracellular matrix degradation in three-dimensional collagen gels

Figure 3

Immunoblot for matrix metalloprotease (MMP)-1. Media were harvested after 5, 10, 15 and 21 days under control conditions and, after five days, in the presence of neutrophil elastase (NE) and were subjected to immunoblot for MMP-1. (a) Human fetal lung (HFL) fibroblasts alone. (b) Blood monocytes (BM) co-cultured with HFL fibroblasts. MMP-1 appears in its latent 52 kDa form. NE presence resulted in conversion of latent MMP-1 to active 42 kDa and 20 kDa forms. (c) BM alone. Lane 1: samples harvested on day 5. Lane 2: culture medium in the presence of NE, harvested at day 5. Lanes 3, 4, 5: samples harvested on days 10, 15 and 21 under control conditions, respectively.

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Respiratory Research

ISSN: 1465-993X

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