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Figure 1 | Respiratory Research

Figure 1

From: Collaborative interactions between neutrophil elastase and metalloproteinases in extracellular matrix degradation in three-dimensional collagen gels

Figure 1

Matrix metalloproteases 2 and 9 (MMP-2 and MMP-9). Culture media were harvested after 5, 10, 15 and 21 days under control conditions and, after 5 days, in the presence of neutrophil elastase (NE) and subjected to gelatin zymography. (a) Human fetal lung (HFL) fibroblasts alone. MMP-2 latent (72 kDa) and active (66 kDa) forms are shown. (b) Blood monocytes (BM) co-cultured with HFL fibroblasts. MMP-9 latent (92 kDa) and active (83 kDa) forms are shown in addition to MMP-2 forms. Lane 1: samples were harvested on day 5. Lane 2: culture medium in the presence of NE, harvested on day 5. Lanes 3, 4, 5: samples were harvested on days 10, 15 and 21 under control conditions, respectively.

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Respiratory Research

ISSN: 1465-993X

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