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Supplementary Figure 2 | Respiratory Research

Supplementary Figure 2

From: Simultaneous identification of GSTP1 Ile105→Val105 and Ala114→Val114 substitutions using an amplification refractory mutation systempolymerase chain reactionassay: studies in patients with asthma

Supplementary Figure 2

Lanes 1, 3, 5, 7 and 9 include the amplified product from Ala114 specific primer, whereas lanes 2, 4, 6, 8 and 10 include the product from the Val114 specific primer. PCR products from either primer give, after digestion with Bsm AI, fragments of either 343, 322, 260 and 73 base pairs for the Ile105 allele, or 322, 260, 250, 93 and 73 base pairs for the Val105 allele. Lane 11, molecular weight markers, pBR HaeIII. PCR products were visualized in 2% agarose gels.

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