Figure 2
From: Myosin heavy chain and physiological adaptation of the rat diaphragm in elastase-induced emphysema
Representative myosin heavy chain (MHC) immunocytochemistry images of an emphysematous diaphragm after co-incubation with anti-laminin antibody and an antibody against one of the adult MHC isoforms, followed by a fluorescein-tagged secondary antibody against the laminin primary and a rhodamine-tagged secondary antibody against the MHC primary antibodies. Each frame shows a serial section stained with a primary antibody against a different adult MHC isoform: I, IIa, IIx, or IIb.