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Figure 2 | Respiratory Research

Figure 2

From: Mechanism of cigarette smoke condensate-induced acute inflammatory response in human bronchial epithelial cells

Figure 2

Effect of CSC treatment on proinflammatory gene expression. NHBEs were exposed to CSC at a concentration of 0.4 μg/ml for 30 min at 37°C. Total RNA was isolated and specific gene expression measured by RT-PCR assay. Target gene expression levels were normalized to the level of GAPDH expression in each reaction and expressed as fold change relative to control. The values are means ± SEM for n = 3. Significant differences at P < 0.05 are indicated by *. CSC = cigarette smoke condensate; NBHE = normal human bronchial epithelial cell; RT-PCR = reverse transcriptase-polymerase chain reaction; SEM = standard error of the mean

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