Figure 7
From: Activation of adherent vascular neutrophils in the lung during acute endotoxemia
Kinetics of NF-κB, CREB and C/EBP nuclear binding activity in adherent vascular lung neutrophils after endotoxin administration to rats. Cells were isolated from control animals or 0.5–48 hours after endotoxin administration. Nuclear extracts (2–5 μg) were analyzed for NF-κB (left panel), CREB (middle panel) or C/EBP (right panel) binding activity. For supershift assays, extracts of cells isolated from rats 2 hours (NF-κB), 0.5 hours (CREB) or 12 hours (C/EBP) after endotoxin administration were pre-incubated with the indicated antibodies. To confirm the specificity of the oligonucleotides, the extracts were pre-incubated for 20 min with an excess of unlabeled NF-κB, CREB and C/EBP competitors (Comp). One representative gel of three separate experiments is presented. C/EBP = CCAAT/enhancer binding protein; CREB = cAMP response element binding protein; CTL = control; NF-κB = nuclear factor-kappa B