Figure 6From: Activation of adherent vascular neutrophils in the lung during acute endotoxemiaEffects of acute endotoxemia on MAP kinase, PI3K and PKB-α expression. Cells were isolated from control animals or 0.5–48 hours after endotoxin administration. Cytoplasmic extracts were prepared and 10 μg protein/well analyzed for p38, phospho-p38 and p44/42 MAP kinase, PI3K and PKB-α expression by western blotting. One representative gel of three separate experiments is shown. CTL = control MAP = mitogen activated protein; PI3K = phosphatidyl inositol 3 kinase; PKB = protein kinase BBack to article page