Figure 5
From: Activation of adherent vascular neutrophils in the lung during acute endotoxemia
Effects of acute endotoxemia on TNF-α mRNA expression in lung neutrophils. Cells were isolated from control animals or 0.5–48 hours after endotoxin administration. Upper panel: Samples were amplified using specific TNF-α primers and run on denaturing polyacrylamide gels. Lower panel: Radioactivity of each amplified TNF-α cDNA band was normalized relative to its corresponding 18S band. CTL = control; TNF-α= tumor necrosis factor alpha